ABSTRACT
Metabolomics is a new study, which use chromatography, mass spectrometry, nuclear magnetic resonance (NMR), capillary electrophoresis (CE) techniques on the cells, organs and other body fluids and metabolites in samples were isolated, purified and testing, re-use bioinformatics tools on the obtained data are analyzed to obtain one or a set of biomarker information. Based on analysis of the literatures in recent years, metabolomics was summarized from history, concept, advantage, methods, application, difficulties and challenges, journals and books, websites, and its application in forensic medicine was forecasted. As a new branch of global system biology, metabonomics developed rapidly, and its perspective on forensic medicine was feasible and very optimistic.
Subject(s)
Humans , Biomedical Research/methods , Chromatography, Liquid/methods , Electronic Data Processing/methods , Forensic Medicine/methods , Forensic Toxicology/methods , Magnetic Resonance Spectroscopy , Mass Spectrometry/methods , Metabolomics/trends , Pattern Recognition, Automated/methods , Specimen Handling/methodsABSTRACT
OBJECTIVE@#To establish the models of postmortem redistribution(PMR) in dogs with epidural anesthesia and to investigate the effect of temperature on the PMR of Bupivacaine.@*METHODS@#Eighteen male dogs were executed by epidural anesthesia with a dose of 5 mg/kg bupivacaine hydrochloride and randomly divided into three groups, room temperature (20-23 degrees C) group, 4 degrees C group and -20 degrees C group. The cardiac blood, peripheral blood, liver and cerebrum were collected at 0, 2, 4, 8, 24, 48, 72, 96, 120h postmortem. The contents of bupivacaine in those samples were analyzed by GC-NPD and GC-MS, the difference among three groups were compared.@*RESULTS@#The bupivacaine PMR of room temperature group was evident and complex in cardiac blood, peripheral blood and cerebrum. The PMR of 4 degrees C group was weaker and slower than that of normal temperature group. The bupivacaine PMR of the -20 degrees C group was the weakest in three groups.@*CONCLUSION@#PMR of bupivacaine will happen in epidural anesthesia death dogs, but it could be delayed or prevent by low temperature storage.
Subject(s)
Animals , Dogs , Male , Analgesia, Epidural , Anesthetics, Local/pharmacokinetics , Brain/metabolism , Bupivacaine/pharmacokinetics , Forensic Toxicology , Gas Chromatography-Mass Spectrometry/methods , Liver/metabolism , Models, Animal , Postmortem Changes , Temperature , Time Factors , Tissue DistributionABSTRACT
Based on the records of carboxyhemoglobin in blood samples stored for recent years, the stability of carboxyhemoglobin in these samples could be affected by the containers, the storage temperatures, the volumes of air above the blood, the saturation of the initial carboxyhemoglobin and preservatives added in these blood samples, among which the storage temperatures, the volumes of air above the blood and the saturation of the initial carboxyhemoglobin are the major influence factors.